Contact an Account ManagerRapid automated determination of D-penicillamine in plasma and urine by ion-exchange HPLC with electrochemical detection using a gold electrode
Summary
Type: HPLC
Application #: 107600
Catalog#: 720097.40 - MN HPLC Column, Nucleosil SA, 100A, 5um, 4x250mm
Detection: electrochemical, working electrode gold, reference electrode Ag/AgCl, auxiliary electrode glassy carbon, polarization voltage +800mV
Conditions: eluent: 0.02M diammonium hydrogen citrate adjusted to pH 2.2 with phosphoric acid
flow rate 1.5ml/min
pressure 150bar
Authors: Kreuzig F
Source: J Chromatogr 218 (1981) 615 - 620
Details
Legend: Fig. 2. Determination of D-penicillamine in plasma, A) standard (1µg/ml), B)
sample, after five samples a standard is injected. Fig. 3. Separation of 1.
L-cysteine, 2. L-glutathione and 3. D-penicil